One of the objectives of the SELFDOTT EU project (FP7 GA 212797, From capture based to SELF-sustained aquaculture and Domestication of bluefin tuna, Thunnus thynnus) is to determine convenient rearing conditions for bluefin tuna larvae. Besides conventional parameters assessing the quality of the development of larvae (survival, growth), it has been shown in different fish species that onset and activity pattern of some digestive enzymes along development are informative indicators of physiological status of larvae, including nutritional status. Additionally, antioxidant enzymes such as catalase (CAT) are well known to be indicators of biotic stress (pathology, pollutants…) in all organisms and especially those related to diet change or metamorphosis in fish larvae. The expression of the genes coding for these enzymes was studied in tuna larvae from three rearing systems in order to assess and compare the physiological status of the individuals reared with different techniques.