Deep sequencing for de novo construction of a marine fish (Sparus aurata) transcriptome database with a large coverage of protein-coding transcripts

The gilthead sea bream (Sparus aurata) is the main fish species cultured in the Mediterranean area and constitutes an interesting model of research. Nevertheless, transcriptomic and genomic data are still scarce for this highly valuable species. A transcriptome database was constructed by de novo assembly of gilthead sea bream sequences derived from public repositories of mRNA and collections of expressed sequence tags together with new high-quality reads from five cDNA 454 normalized libraries of skeletal muscle (1), intestine (1), head kidney (2) and blood (1).

Autor: 
CALDUCH-GINER, J.A., BERMEJO-NOGALES, A., BENEDITO-PALOS, L., ESTENSORO, I., BALLESTER-LOZANO, G., SITJÀ-BOBADILLA, A., J. PEREZ-SANCHEZ, J.
Referencia: 
BMC Genomics
Volumen: 
14:170