PROFILING OF INFECTION SPECIFIC MRNA TRANSCRIPTS OF THE EUROPEAN SEABASS DICENTRARCHUS LABRAX.

Here we describe the construction, analysis and comparison of a total of ten cDNA libraries of the European seabass (Dicentrarchus labrax), six from different tissues infected with V. anguillarum (liver, spleen, head kidney, gill, peritoneal exudates and intestine) and four cDNA libraries from different tissues infected with Nodavirus (liver, spleen, head kidney and brain). In total 9605 sequences representing 3075 (32%) unique sequences were obtained and analysed. Among the sequences several immune-related proteins were identified for the first time in the order of Perciformes as well as in Teleostei. Some of the potentially interesting genes identified by in silico analysis and confirmed by real-time PCR are putative biomarkers for bacterial and viral infections in fish.