NEW TETRACHROMIC VOF STAIN (TYPE III-G.S) FOR NORMAL AND PATHOLOGICAL FISH TISSUES

A new VOF Type III stain was applied to histological sections of different organs and tissues of healthy and pathological larvae, juvenile and adult fish species (solea senegalensis, Sparus aurata, diplodus sargo, Pagrus auriga, Argyrosomus regius and Halobatrachus didactylus. In comparison to original Gutierrez VOF stain, more acid dyes of contrasting colours and plychromatic/metachromatic properties were incorporated as essential constituents of the tetrachromic VOF stain. This facilites the selective staining of different basic tissues and improves the morphological analysis of histochemical approaches of the cell components. The VOD Type III stain is composed of a mixture of several dyes of varyiing size and molecular weight (orange G< Acid Fuchsin< Ligh Green