In this study we applied the Fur titration assay (FURTA) to isolate iron regulated genes in Aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis in fish. We have identified genes for siderophore biosynthesis and for ferri-siderophore transport, some of which have been previously described in this species. A gene for a hitherto uncharacterized functional hemin receptor HutE was identified, but its inactivation did not affect significantly the use of hemin as sole iron source, suggesting that redundant genes encoding hemin receptors exist in the A. salmonicida genome. Additional FURTA positive clones contained genes encoding a Flavoprotein, a transcriptional regulator protein, a DNA binding protein Fis, as well as genes encoding putative DapD and Flp/Fap-pilin proteins. A screening of gene distribution demonstrated that all the analyzed strains shared genes for siderophore biosynthesis and transport and for heme utilization, indicating that these two systems of iron acquisition are a conserved trait in this subspecies, whereas other genes are not common to all the isolates. Thus, the Fur regulon of A. salmonicida subsp. salmonicida includes both conserved and differentially occurring genes, suggesting that the genetic diversity within this fish pathogen might be higher than expected.
