A method is described for the rapid and sensitive assay of phagocytosis that utilizes radioactively labelled bacteria. With this method, we observed that phagocytosis of heatinactivated Aeromonas salmonicida by leukocytes isolated from gut of rainbow trout fed with different viable probiotics (Lactococcus lactis subsp. lactis, Lactobacillus sakei, and Leuconostoc mesenteroides) was significantly higher (Po0.05) after 2 weeks of probioticfeeding than the control group. However, only phagocytosis of live A. salmonicida by the leukocytes isolated from gut of rainbow trout fed with L. lactis subsp. lactis was significantlyhigher (P>0.05) than the control group.
Autor:
Balc?zar JL, Vendrell D, de Blas I, Ruiz-Zarzuela I, Giron?s O, Muzquiz JL
Referencia:
Comparative Immunology, Microbiology and Infectious Diseases
Volumen:
29
Pagina Inicial:
335
Pagina final:
343
Editorial:
Elsevier